Gene description for RAB5C
Gene name RAB5C, member RAS oncogene family
Gene symbol RAB5C
Other names/aliases L1880
RAB5CL
RAB5L
RABL
Species Homo sapiens
 Experiment description of studies that identified RAB5C in Breast milk

1
Experiment ID574
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry [LTQ-Orbitrap Elite, Q-Exactive]
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components
AuthorsVan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name MCP
Publication year2016
SampleBreast milk
Sample nameBreast milk - normal
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV160000: EV-METRIC:50%

2
Experiment ID652
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28290293   
OrganismHomo sapiens
Experiment descriptionComparative proteomic analysis of milk-derived exosomes in human and bovine colostrum and mature milk samples by iTRAQ-coupled LC-MS/MS.
AuthorsYng M, Song D, Cao X, Wu R, Liu B, Ye W, Wu J, Yue X.
Journal name Food Res Int
Publication year2017
SampleBreast milk
Sample nameColostrum
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Filtration
Density gradient centrifugation
Flotation density1.7 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

3
Experiment ID653
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28290293   
OrganismHomo sapiens
Experiment descriptionComparative proteomic analysis of milk-derived exosomes in human and bovine colostrum and mature milk samples by iTRAQ-coupled LC-MS/MS.
AuthorsYng M, Song D, Cao X, Wu R, Liu B, Ye W, Wu J, Yue X.
Journal name Food Res Int
Publication year2017
SampleBreast milk
Sample nameMature milk
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Filtration
Density gradient centrifugation
Flotation density1.7 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

4
Experiment ID950
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components.
Authorsvan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name Mol Cell Proteomics
Publication year2016
SampleBreast milk
Sample nameMilk - derived EVs - D1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV160000: EV-METRIC:50%

5
Experiment ID951
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components.
Authorsvan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name Mol Cell Proteomics
Publication year2016
SampleBreast milk
Sample nameMilk - derived EVs - D2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV160000: EV-METRIC:50%

6
Experiment ID952
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components.
Authorsvan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name Mol Cell Proteomics
Publication year2016
SampleBreast milk
Sample nameMilk - derived EVs - D3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV160000: EV-METRIC:50%

7
Experiment ID953
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components.
Authorsvan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name Mol Cell Proteomics
Publication year2016
SampleBreast milk
Sample nameMilk - derived EVs - D4
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV160000: EV-METRIC:50%

8
Experiment ID954
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components.
Authorsvan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name Mol Cell Proteomics
Publication year2016
SampleBreast milk
Sample nameMilk - derived EVs - D5
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV160000: EV-METRIC:50%

9
Experiment ID955
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components.
Authorsvan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name Mol Cell Proteomics
Publication year2016
SampleBreast milk
Sample nameMilk - derived EVs - D6
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV160000: EV-METRIC:50%

10
Experiment ID956
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components.
Authorsvan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name Mol Cell Proteomics
Publication year2016
SampleBreast milk
Sample nameMilk - derived EVs - D7
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV160000: EV-METRIC:50%

11
Experiment ID957
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components.
Authorsvan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name Mol Cell Proteomics
Publication year2016
SampleBreast milk
Sample nameMilk - derived EVs - pooled
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV160000: EV-METRIC:50%

12
Experiment ID2212
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 37043568   
OrganismHomo sapiens
Experiment descriptionMilk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis
AuthorsTong L, Zhang S, Liu Q, Huang C, Hao H, Tan MS, Yu X, Lou CKL, Huang R, Zhang Z, Liu T, Gong P, Ng CH, Muthiah M, Pastorin G, Wacker MG, Chen X, Storm G, Lee CN, Zhang L, Yi H, Wang JW.
Journal name Sci Adv
Publication year2023
SampleBreast milk
Sample nameColostrum
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Centrifugal ultrafiltration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

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